In view of the limitations of the traditional fundus camera with poor mobility, complicated equipment and image transmission limited by wiring and object pose, a wireless retinal imaging system based on WiFi wireless local area network is designed. The system mainly consists of three components, including the imaging system, PC software operating system, and image transmission system. A hand-held fundus camera realizes real time images acquisition, and displays the image information on the PC side through wireless transmission mode, to achieve real-time observation and preservation of the fundus image and as an objective basis for the diagnosis and treatment of ophthalmic diseases. Finally, the examination results will be stored in the database to establish electronic medical records with relational database SQL Server 2008, so that the results can achieve the functions like archiving, accessing, and sharing for the diagnosis of retinal disease in remote medical and large data mining applications.