Chinese word segmentation technology is the most critical part of Chinese word processing technology, and it is widely used as one of the most important part in pre-processing methods. This paper mainly analyses and compares the influence of ASPSeek, ICTCLAS, Paoding, MMseg, IK and JE six segmentation tools on agricultural search engine. The results showed that ICTCLAS word segmentation has the most optimization in accuracy, while JE word segmentation has the highest measure of F1 in the application effect of agricultural search engine.