Inverse sub-structure dynamic analysis of structural dynamics is a technical theory and analysis method recently developed, but it requires large amounts of data for analysis and calculation. In order to analysis efficiently, here comes the development of a indirect inverse sub-structure dynamic quality detection software. Calculated based on the the indirect inverse sub-structure theory, based on the indirect inverse sub the discretization packaging coupled dynamic stiffness structural analysis calculation method and mode shapes combined method. Using Matlab graphical user interface (GUI), data importing, processing, mapping and other operations can be easily implemented, while the use of Matlab powerful matrix computing power to quickly calculate the required results for professionals to analyze.