The introduction of this paper is based on STM32 and μC/OS-II for ECG data detection system design and implementation. Using STM32F130ZET6 as the microprocessor, with TFT LCD screen Graphical display module and SD card data storage/read and write module and so on, and usingμC/GUI for managing graphical interface. The system implements the acquisition and processing of ECG signals, displaying in real-time on the LCD screen, control of touch screen, and storing the data in the SD card in order to transfer the date to PC and other functions.