According to the traditional method of inspection antibiotic residues on seafood, it exists Labor-intensive and highly Repetitive, this article researched and established the automatic inspection system of antibiotic residues based on computer vision. The system include the image of inhibition zone's adaptive de-noise and background correction, quadratic bacteriostatic ring fitting based on multiresolution, the compute method of antibiotic residues which meets the national standards. The antibiotic residues inspection of seafood tetracyclines show that the method in this article has the advantages like high speed, high accuracy and easy to operate etc. We developed the system is enough to satisfy the demand of partial antibiotic residues' inspection. Theory and experimental results have shown the system's advantages and effectiveness.