Amblyopia is a developmental visual disorder characterized by reduced vision in the absence of any detectable structural or pathological abnormalities that can not improve with refractive correction, there are about 2.8% of people in China have amblyopic disease. Amblyopia is a developmental disease , it will be better to detect and treat as soon as possible, therefore, according to the mechanism of amblyopia, use psychophysical methods, design an examination method to help doctors to diagnose the disease earlier, and make better treatment subjects for the patients will have important meaning. In this paper, Matlab7.0 and Psychophysics Toolbox2.5 are adopted to design and implement a new method based on Gabor stimulus which used for detecting contrast sensitivity and crowding effect in amblyopia. The clinical trails show that the examination method is simple and convenient, it has important meaning to detect and examin different vision functions of amblyopia.