Radionuclide imaging agent is necessary for PET/CT imaging. At present, the dispensing method of radionuclide imaging agent is mainly traditional manual dilution dispensing, so there are problems such as low efficiency of dispensing, large dose of radiation to human body, and so on. In order to solve the above problems, the researchers have worked on the automatic dispensing system, but the function of the control interface is single, and it cannot monitor the dispensing work in real time. So this study designed an automated Dispensing Hot Cells (DHC) control interface based on Qt. This interface can receive the data through the network transmission of remote cameras to realize real-time video display of the internal situation of the DHC and used the brightness equalization algorithm to process the image. And the interface uses the serial port protocol to connect the STM32F429 demo board, designed the corresponding functional controls to operate the demo board, realizing the automation control of DHC with the dispensing mechanical system. The verification shows that, this interface realizes the friendly interface function of human-machine interaction. It improves the quality of monitoring and the response speed of the system. Compared with the commonly used monitoring software MiniVCap and VCam, the video delay and the CPU occupancy rate have been significantly reduced, can be stably connected with STM32F429, and meet the design requirements in terms of performance and indexes.