In the operation room, the traditional human-computer interaction technology relies heavily on the touch-based devices such as mouse, keyboard, and touch screen, which raises the risk of infection during the surgery. Human-computer interaction based on gesture recognition has a great advantage in the cost and interactive naturalness. This paper introduces a gesture-based control technology utilizing Leap Motion, the use of AR technology and Leap Motion equipment make doctors do not need to touch the surgical equipment during surgery. With such help of non-contact equipment, doctors can be completely away from the interference and surgical risk because of the contact to the equipment during surgery. The Leap Motion based interaction technology provides the possibility of reducing the risk of surgical infection in the operation room.