Amblyopia is a developmental disorder of the visual system. There are about 2.8% of people in China who have amblyopic disease. The examinations of visual functions for amblyopia can help doctors make better treatment for patients. In this paper, a system with a psychophysics method is introduced to examine different visual functions of amblyopia. For the software system, MFC is used in the user interface and the overall framework. The Psychophysics Toolbox based on Matlab is adopted to realize the model of visual functions examination. SQL Server is used to generate back-end database. Meanwhile, a hybrid programming system between VC++ and Matlab is adopted to combine the entire system into one. For the hardware of the system, a Vedio-Switcher is used to improve the display accuracy to meet the special requirements of the vision test. The results show that this system has good stability and can meet the needs of visual functions examination for amblyopia.