This study proposes a highly trusted interaction framework for business behavior control based on network isolation architecture. This framework not only makes the access of business data in the high security zone under the complex security architecture for enterprise internet mobile applications possible but also ensures the security of key data of the business system. Under the requirements of network security protection, the mobile access gateway is introduced to decompose the interaction process of business data across the security zone. Then, it designs an access conversion and communication method, which realizes the safe and reliable transmission of business data through various isolation devices and business flow control. The framework has been widely used in many business areas such as employee reimbursement, attendance punching, power system distribution repair and mobile inspection.