When the sensor nodes collect data in the forest or water environment, the signal intensity and the signal transmission speed are affected by the obstacles or transmission media, which affects the measurement accuracy of localization algorithm based on the signal information. Taking into account the cost of nodes and the dynamic nature of nodes, DV-Hop positioning algorithm has excellent applicability and practicality. This paper analyses the factors influencing the accuracy of DV-Hop algorithm, proposes a formula for measuring the degree of uniformity of nodes, optimized the algorithm. The simulation results show that the optimized algorithm has higher positioning accuracy.