This paper presents the design of a serial communication software cross network transmission, the software uses the data transceiver double thread mechanism and data queue data sharing mechanism between the threads, in order to ensure the data security efficiency and integrity, those methods are designed on the data communication, such as data frame type check, data frame CRC check and data frame length check. Through the test, the rate and bit error test, it proves that this model to realize serial communication is reliable, safe and efficient, to meet the future real-time data communication between the mission network and the information networks needs to be.