Multiple input multiple output-orthogonal frequency division multiplexing MIMO-OFDM technology is the core of the next generation(4G) of communication technology. MIMO-OFDM has the advantages of high spectrum efficiency, strong anti-interference capability and large Channel capacity. Distributed MIMO-OFDM system can produce time delay and frequency offset. It is also very sensitive to the timing and frequency offset. So, the study of distributed MIMO-OFDM synchronization algorithm is more practical significance. The article made a comprehensive analysis and summary from the sequence structure and the performance of these three current kinds of distributed MIMO-OFDM timing synchronization algorithm, and through the MATLAB simulation comparison. It acquires to use CAZAC sequence as subcarrier data reverse conjugate timing algorithm has outstanding performance.