Virtual electronic experiment system is designed based on LabVIEW, including foundation and comprehensive experiment of analog circuit and digital circuit, mainly uses analog circuit experiment simulation based on LabVIEW and Multisim Co-Simulation, make full use of digital control of LabVIEW and analog simulation of Multisim. Meanwhile, the digital circuit experiments are projected by LabVIEW, bringing about opening, scalability, sharing resources of virtual instrument development platform, and also illustrates the design process of system modules by examples. Virtual electronic experiment system based on LabVIEW and Multisim not only can effectively alleviate the shortage of laboratory equipment conditions, and save the input of laboratory, but also provide new ideas of deepen the teaching reform.