LabVIEW and MATLAB are two kinds of popular software that widely used in the engineering project technical, by calling the MATLAB in LabVIEW to implement the seamless links, and make full use of Matlab’s rich functions, strong scientific computing and data processing ability, complement each other, which can greatly improve the development of the virtual instrument function. aslo the LabVIEW by calling in the MATLAB Script node is introduced in detail. Finally, Realized the seamless links between LabVIEW and MATLAB, using two examples shows the superiority of feasibility of LabVIEW and MATLAB seamless links as well as the two complementary advantages.