This paper mainly studies the target positioning system based on nanoLOC transceiver. An embedded positioning system consists of S3C6410A microprocessor of Samsung, nanoPAN 5375 wireless communication module and LCD. Linux operating system based on Linux 2.6.38 kernel is used as embedded software platform. The symmetrical double-sided two-way ranging algorithm is designed which has a high accuracy of positioning system. A lot of experiments show that the accuracy of positioning system is less than one meter, the system can achieves in indoor and outdoor as well as the high precision positioning in harsh environments, and be used in handheld mobile positioning equipment. The system has a great application and promotion value.