The Dissolved Oxygen (DO) control system for rice wine fermentation is an unstable system with multi-disturbance, time-varying and nonlinearity. A novel DO control method is proposed. The system is based on Labview, SIMATIC PLC300 and sensors, the Kalman filtering and Genetic PID control strategy are introduced. The PID control parameters are tuned by genetic algorithm on line, while a Kalman filter is used to reduce the impact of control interference and noise on system. Finally, a simulation experiment is performed by Matlab, and simulation result shows that the control method not only has a good adaptability and Anti-disturbance but also has strong robust ability and fast convergent rate.