In multi-terminal Java ME Bluetooth serial port communications, it is necessary to access and control remote file directory structure. To ensure that under the conditions of serial connections it is convenient and real-time to access the directory structure information, and locate the file accurately, the directory structure must also be controlled dynamically. To address these problems, this paper researches and designs the two-dimensional vector storage structure and access control methods, along with the application and the realization of the method in the Bluetooth serial port communication. The results of practical application show the effectiveness of dynamic access control.