Abstract:In consideration of the problem that useful information cannot be easily extracted from various types of long noncoding RNA (lncRNA) data, this paper proposes a visualization system of multi-source lncRNA data based on generic genome browser (GBrowse). The system mainly includes a web server including HTTP service and GBrowse components, and lncRNA data storage which supports flat files, MySQL, SQLite and other types of databases. The main steps of constructing the system include GBrowse installation and configuration, multi-source lncRNA data collection, preprocessing, storage, and access and visualization configuration. A demo system is constructed by firstly collecting six sets of human lncRNA data, including human gene annotation, genome sequence, histone modification H3K4me3 signals and their loci predicted, signals of transcription factor CTCF binding sites and their loci predicted. After preprocessing, these data are stored by databases such as MySQL, SQLite and so on, and data access and visualization methods are also configured. The experiment results demonstrate that multi-source lncRNA data can be integrated and visualized within the GBrowse framework, and be showed in the genome spatial space simultaneously, which can make researchers observe the lncRNA data more intuitively, thereby helps to produce novel scientific hypothesis.