This paper mainly presents a virtual infrared scalpel with several techniques including OpenGL and 3DMax, stereoscopic vision and the quaternion. Firstly, it obtains the 3-d information of the virtual surgical knife with infrared light using the stereoscopic and infrared tracking. Secondly, it reflects the information to the scalpel modeling based on the OpenGL and 3DMax and rotates with the quaternion. Finally, it builds a virtual infrared scalpel. Experiment result demonstrates that the system can be applied to the surgery operation effectively for the interactive processing.