The introduction of this paper is based on MinGui and SD card FAT32 file system for data acquisition and display terminal design and implementation. Using SEP4020 as the microprocessor, with AD data acquisition module, TFT LCD screen Graphical display module and SD card data storage / read and write module ect, and using μC / OS-II embedded operating system for managing switching and communication between tasks. The system implements the acquisition of analog and digital signals, displaying in real-time on the terminal display, operatoring control of touch screen, and storing the data on the SD card for the PC for further data analysis and other functions.