Focused on the issue of the complexity and uncertainty of high-altitude inspection environment, a semantic interaction method of UAV navigation for transmission line inspection is proposed. Firstly, a semantic topology of human-UVA interaction based on ontology is structed to form a navigation framework with perceptual motion data processing. Then, an algorithm of structured route navigation is proposed by combining the background knowledge of predicated logic form and semantic topology based. Lastly, a semantic interaction demonstration system is developed and the ideal results of the experimental are obtained. As a case study of semantic interaction, this research proves that the entity noun extraction satisfies actual expectation estimation and has high precision control, which can provide high position navigation control UAV transmission line inspection. It also can provide basic support for data-driven transmission line inspection.