For the problem of deblurred confocal image restoration under Poisson noise, a regularization method based on Hessian matrix norm was proposed to solve the stairs effect existing in traditional methods. Based on the Poisson probability model, the method introduced the Hessian matrix norm as a regular condition, and applied the alternating direction multiplier method and gradient projection method to solve the optimization model. The quality of the reconstructed image obtained in the confocal laser scanning microscopy experiment was better than that of the traditional method. This result proves that the method can effectively restore the deblurred confocal image under Poisson noise.